This method exhibits good specificity and high sensitivity with a limit of detection (LOD) of 1.75 × 10 −6 U μL −1. The ssRNA can hybridize with a molecular beacon to form an RNA/DNA heteroduplex that can be selectively digested by DSN to generate an enhanced fluorescence signal. Upon the addition of T7 RNA polymerase, an efficient T7 transcription amplification reaction is initiated to produce abundant single-stranded RNAs (ssRNAs). The ssDNA can hybridize with the T7 promoter-bearing template probe to trigger the extension with the aid of Klenow fragment (KF) DNA polymerase. In the presence of FEN1, the flapped dumbbell probe is cleaved to generate a free 5′ flap single-stranded DNA (ssDNA) with the 3′-OH terminus. Herein, we develop a target-activated T7 transcription circuit-mediated multiple cycling signal amplification platform for monitoring FEN1 activity in cancer cells. You will need to install the Ubisoft Connect for PC application in order to run this content.The structure-specific endonuclease flap endonuclease 1 (FEN1) is an essential functional protein in DNA replication and genome stability, and it has been identified as a promising biomarker and drug target for multiple cancers. PC Game contains VMProtect ( ) anti-tamper technology.Īutomatically added to your Ubisoft Connect for PC library for download. SHARE EVERYTHING - Capture your most insane stunts and share via social media. PUT YOUR SKILLS TO THE TEST - Prove that you're king of the mountain in unique and spectacular challenges. THE MOUNTAIN IS YOURS TO EXPLORE - Create your own path through the massive open-world of the Alps and Alaska, and share your best custom lines with friends. LIVE UNFORGETTABLE MOMENTS WITH OTHERS - Ride solo or drop in next to other players to share thrilling, adrenaline-fueled rides.
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Dare your friends to try out your custom lines, then challenge the world to beat your best tricks and relive your most epic wipeouts. Record and share the most insane stunts ever captured. Go solo or drop-in side by side with other players. Ride the massive open world of the Alps and Alaska, where the powder is always fresh and the run never ends.ĭefy and master the world’s most epic mountains on skis, wingsuits, snowboards, and paragliders.
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